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Study Guide: Endosymbiosis: Mechanisms, Examples, and Evolutionary Significance

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Endosymbiosis: Mechanisms, Examples, and Evolutionary Significance Study Guide

Foundations of Endosymbiosis

Endosymbionts are organisms that exclusively inhabit the external environment of a host organism.

Answer: False

Explanation: This statement is incorrect. Endosymbionts, by definition, live *within* the body or cells of another organism. Organisms living exclusively outside are typically considered ectosymbionts or free-living.

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The symbiotic relationship between an endosymbiont and its host is predominantly characterized by parasitism.

Answer: False

Explanation: This assertion is inaccurate. While parasitic relationships exist, the majority of endosymbiotic associations are mutualistic, where both the host and the symbiont derive benefits.

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An obligate endosymbiont is one that can survive independently of its host.

Answer: False

Explanation: This statement is incorrect. An obligate endosymbiont is defined by its absolute dependence on the host organism for survival. Facultative endosymbionts are those capable of independent existence.

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The term 'endosymbiont' is derived from Latin roots meaning 'inside', 'together', and 'life'.

Answer: False

Explanation: The term 'endosymbiont' originates from Greek roots: 'endon' (within), 'syn' (together), and 'biosis' (living). Latin roots are not the primary source for this term.

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A holobiont consists solely of a host organism and its genetic material.

Answer: False

Explanation: This definition is incomplete. A holobiont encompasses the host organism *and* all of its associated microbial symbionts, functioning as an integrated unit.

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Define the term 'endosymbiont' with respect to its ecological niche and relationship with a host.

Answer: An organism that resides within the body or cells of another organism.

Explanation: An endosymbiont is an organism that lives within the body or cells of another organism, forming a symbiotic association.

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Which of the following scenarios represents a commonly cited example of an endosymbiotic host-symbiont relationship?

Answer: Unicellular algae residing within the tissues of reef-building corals.

Explanation: The mutualistic association between corals and their endosymbiotic dinoflagellates (zooxanthellae) is a classic and widespread example of endosymbiosis, crucial for coral reef ecosystems.

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What is the primary characteristic of a facultative endosymbiont?

Answer: It can survive independently of its host organism.

Explanation: A facultative endosymbiont is defined by its ability to exist independently outside of a host organism, although it may also live symbiotically within a host.

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What term describes a host organism and its symbiotic microorganisms, particularly when the symbionts are highly dependent and resemble cellular organelles?

Answer: Holobiont

Explanation: The term 'holobiont' refers to the host organism and all of its associated microbial symbionts, viewed as a single ecological and evolutionary unit.

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Mechanisms and Transmission

As an endosymbiont becomes more integrated with its host, its genome typically expands to include more host genes.

Answer: False

Explanation: This is contrary to observed patterns. As endosymbionts become more integrated, their genomes typically undergo reduction, losing genes that are redundant or provided by the host, rather than expanding.

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Horizontal symbiont transfer occurs when a symbiont is passed directly from a parent organism to its offspring.

Answer: False

Explanation: This statement is incorrect. Horizontal symbiont transfer involves the acquisition of symbionts from the external environment or other hosts, distinct from vertical transmission, which is parent-to-offspring inheritance.

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Vertical symbiont transmission requires the symbiont to be able to survive independently in the external environment.

Answer: False

Explanation: This statement is incorrect. Vertical transmission ensures symbionts are passed directly to offspring, often reducing the need for independent survival and potentially leading to genome reduction, rather than requiring it.

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Cospeciation, where endosymbionts speciate in parallel with their hosts, is commonly observed with secondary endosymbionts.

Answer: False

Explanation: Cospeciation is more commonly associated with primary endosymbionts, which typically have long-term co-evolutionary relationships with their hosts, rather than secondary endosymbionts that are often acquired more recently and are not obligate.

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Genome reduction in endosymbionts like *Buchnera* occurs because they rely on the host for certain functions and live in a stable environment.

Answer: True

Explanation: This statement accurately describes the evolutionary pressures leading to genome reduction in obligate endosymbionts: the availability of host-provided resources and functions, coupled with the reduced selective pressure for maintaining genes related to independent survival.

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What typically happens to the genome of an endosymbiont as it becomes more integrated with its host?

Answer: The genome shrinks as genes are discarded.

Explanation: Obligate endosymbionts often experience genome reduction. This process involves the loss of genes that are no longer essential for survival because the host provides the corresponding functions or molecules, leading to a streamlined genome.

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Which mode of symbiont transmission involves acquiring the symbiont from the environment or other hosts?

Answer: Horizontal transmission

Explanation: Horizontal transmission refers to the acquisition of symbionts from sources external to the host's lineage, such as the environment or other individuals, as opposed to direct inheritance from parents.

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What is a consequence for an endosymbiont that is transmitted vertically?

Answer: It often does not need to survive independently, potentially leading to genome reduction.

Explanation: Vertical transmission ensures a consistent supply of symbionts to offspring. This can reduce the selective pressure for independent survival, potentially leading to the loss of genes required for free-living existence and subsequent genome reduction.

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Cospeciation refers to:

Answer: The process where parasites or endosymbionts speciate in parallel with their hosts.

Explanation: Cospeciation describes the simultaneous evolutionary divergence of host and symbiont lineages, indicating a long-standing and tightly co-evolved relationship.

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Genome reduction in endosymbionts like *Buchnera* is primarily driven by:

Answer: The host cell providing essential functions and a stable environment.

Explanation: The stable intracellular environment and the host's provision of essential metabolites reduce the selective pressure for maintaining genes related to independent survival, leading to genome streamlining.

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How can endosymbionts potentially influence the evolution of their hosts?

Answer: By providing new metabolic capabilities and altering physiology.

Explanation: Endosymbionts can introduce new metabolic pathways (e.g., nutrient synthesis) or alter host physiology, providing adaptive advantages that drive host evolution, potentially leading to niche expansion or diversification.

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Which statement accurately describes the difference between obligate and facultative symbionts regarding transmission?

Answer: Obligate symbionts require the host and are typically transmitted vertically; facultative symbionts can survive independently and are often transmitted horizontally.

Explanation: Obligate symbionts are intrinsically dependent on their host and usually rely on vertical transmission for propagation. Facultative symbionts, capable of independent survival, are often acquired horizontally from the environment.

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Evolutionary Origins

Chloroplasts in plant cells are believed to have originated from the engulfment of archaea over a billion years ago.

Answer: False

Explanation: The prevailing endosymbiotic theory posits that chloroplasts originated from the engulfment of cyanobacteria, not archaea, approximately 1 to 1.5 billion years ago.

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The symbiogenesis theory suggests that eukaryotic cells evolved through the absorption of prokaryotes, leading to the development of organelles.

Answer: True

Explanation: This statement accurately reflects the core tenet of the symbiogenesis theory, which proposes that organelles like mitochondria and chloroplasts originated from free-living prokaryotes that were engulfed by host cells and established enduring endosymbiotic relationships.

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According to the endosymbiotic theory, what type of organism is believed to have evolved into mitochondria?

Answer: A bacterium

Explanation: The endosymbiotic theory posits that mitochondria originated from an aerobic bacterium that was engulfed by an early eukaryotic precursor cell.

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The symbiogenesis theory explains the origin of:

Answer: Eukaryotic cells

Explanation: Symbiogenesis is the leading theory explaining the origin of eukaryotic cells, proposing that complex cellular structures and organelles arose from symbiotic relationships between different prokaryotic organisms.

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Endosymbiosis in Insects

Primary endosymbionts in insects are typically associated with their hosts for only a short evolutionary period.

Answer: False

Explanation: This statement is accurate. Primary endosymbionts (P-endosymbionts) often exhibit long-term co-evolutionary histories with their insect hosts, sometimes spanning millions of years, which can lead to phenomena like cospeciation.

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The *Buchnera* endosymbiont, found in pea aphids, is known for synthesizing essential vitamins for its host.

Answer: False

Explanation: This statement is incorrect. *Buchnera* primarily synthesizes essential amino acids that are deficient in the aphid's plant sap diet, not essential vitamins.

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*Hamiltonella defensa*, a secondary endosymbiont, protects pea aphids by producing toxins that harm parasitoid wasps.

Answer: True

Explanation: This statement is correct. *Hamiltonella defensa* is known to provide a defensive benefit to pea aphids by producing toxins that incapacitate or kill parasitoid wasps attempting to lay eggs in the aphid.

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Which primary endosymbiont is known for providing essential vitamins to its tsetse fly host?

Answer: *Wigglesworthia*

Explanation: *Wigglesworthia* is an obligate primary endosymbiont of the tsetse fly, essential for providing vital vitamins that the fly cannot obtain from its blood-based diet.

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Secondary endosymbionts in insects, unlike primary endosymbionts, are often:

Answer: Acquired horizontally and are not obligate.

Explanation: Secondary endosymbionts are typically acquired horizontally from the environment and are not obligate for host survival, distinguishing them from primary endosymbionts which are often vertically transmitted and essential.

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What is the proposed role of *Spiroplasma poulsonii* as a defensive symbiont?

Answer: It protects *Drosophila* flies against nematode parasites.

Explanation: *Spiroplasma poulsonii* has been shown to confer protection to *Drosophila* flies against nematode infections by producing toxins that target the parasites.

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What is the primary function of *Wigglesworthia* in tsetse flies?

Answer: Providing essential vitamins.

Explanation: *Wigglesworthia* is an obligate endosymbiont crucial for synthesizing essential vitamins that the tsetse fly cannot obtain from its blood diet.

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What is a potential application of studying insect endosymbionts?

Answer: Formulation of innovative strategies for controlling insect pests and disease vectors.

Explanation: Understanding the dependencies of insect pests or disease vectors on their essential endosymbionts can inform the development of targeted control strategies, such as disrupting symbiont transmission or function.

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Endosymbiosis in Plants and Fungi

Rhizobia bacteria, which fix nitrogen, are commonly found as endosymbionts in the root nodules of legumes.

Answer: True

Explanation: This statement is correct. The symbiotic relationship between Rhizobia bacteria and legumes, where the bacteria reside within root nodules and fix atmospheric nitrogen, is a well-established example of endosymbiosis.

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Endophytes can assist vascular plants by enhancing growth, nutrient uptake, and defense against herbivores.

Answer: True

Explanation: This statement accurately reflects the multifaceted benefits endophytes can confer upon their vascular plant hosts, encompassing physiological support and protective functions.

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All plant endosymbionts are beneficial or mutualistic in their relationship with the host plant.

Answer: False

Explanation: This assertion is incorrect. While many plant endosymbionts are mutualistic or commensal, some can be pathogenic or exhibit conditional benefits, meaning their impact can vary depending on environmental factors or host status.

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Arbuscular Mycorrhizal Fungi (AMF) primarily help plants by providing them with carbon compounds from the soil.

Answer: False

Explanation: AMF primarily help plants by providing them with soil nutrients, like phosphorus and nitrogen, in exchange for carbohydrates produced by the plant through photosynthesis.

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Plants attract Arbuscular Mycorrhizal Fungi (AMF) by releasing strigolactones and flavonoids from their leaves.

Answer: False

Explanation: Plants attract AMF by releasing chemical signals, such as strigolactones and flavonoids, from their roots, not their leaves.

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Endophytic fungi typically live exclusively on the outer surface of plant tissues.

Answer: False

Explanation: Endophytic fungi colonize the internal tissues of plants, residing within host cells or intercellular spaces, rather than solely on the exterior.

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'True' endophytes are characterized by colonizing plant tissues through random events, lacking specific adaptive traits.

Answer: False

Explanation: True endophytes possess specific adaptive traits that enable consistent association with host plants, unlike 'passenger' endophytes which colonize randomly.

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The Rhizobia-Legume symbiosis is initiated when the legume host releases which type of chemical signal?

Answer: Flavonoids

Explanation: Legume hosts release flavonoids from their roots, which act as chemoattractants and signal molecules that induce the expression of *Nod* genes in Rhizobia, thereby initiating the nodulation process.

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Which of the following is a function of endophytes in vascular plants?

Answer: Facilitating growth, nutrient assimilation, and defensive capabilities.

Explanation: Endophytes can significantly benefit their host plants by promoting growth, enhancing the uptake of essential nutrients, and bolstering resistance to environmental stresses and herbivory.

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What do Arbuscular Mycorrhizal Fungi (AMF) receive in exchange for helping plants acquire soil nutrients?

Answer: Organic carbon compounds synthesized via the plant's photosynthesis.

Explanation: AMF colonize plant roots and enhance nutrient uptake, primarily receiving photosynthetically derived carbohydrates (sugars) from the plant in exchange.

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Plants attract AMF endosymbionts primarily by releasing chemical signals from their:

Answer: Roots

Explanation: Chemical signals, such as strigolactones and flavonoids, are released from plant roots to attract AMF, initiating the symbiotic colonization process.

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What distinguishes 'true' endophytes from 'passenger' endophytic bacteria?

Answer: 'True' endophytes possess specific adaptive traits for consistent association, while passengers colonize opportunistically.

Explanation: True endophytes have evolved mechanisms for stable integration and often provide benefits, whereas passenger endophytes colonize plant tissues incidentally without specialized adaptations for long-term association.

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Diverse Examples and Recent Discoveries

Zooxanthellae are bacteria that provide energy to corals through chemosynthesis.

Answer: False

Explanation: This statement contains two inaccuracies: Zooxanthellae are dinoflagellate algae, not bacteria, and they provide energy to corals via photosynthesis, not chemosynthesis.

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In nutrient-poor oceans, endosymbiotic cyanobacteria like *Richelia intracellularis* are significant because they perform photosynthesis for their diatom hosts.

Answer: False

Explanation: This statement is incorrect. While photosynthesis is vital, the primary significance of *Richelia intracellularis* in nutrient-poor oceans lies in its capacity for nitrogen fixation, providing essential nitrogen to its diatom host.

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The protozoan *Mixotricha paradoxa* hosts spherical bacteria that perform photosynthesis within its cytoplasm.

Answer: False

Explanation: This statement is incorrect. The bacteria hosted by *Mixotricha paradoxa* are primarily involved in respiration and methanogenesis, not photosynthesis.

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The chromatophore in *Paulinella chromatophora* is functionally equivalent to traditional mitochondria.

Answer: False

Explanation: This statement is incorrect. The chromatophore, derived from a cyanobacterial endosymbiont, is functionally analogous to chloroplasts, responsible for photosynthesis, not mitochondria which are involved in cellular respiration.

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The study involving *Amoeba proteus* and bacteria demonstrated that the initial infection always resulted in a stable, mutually dependent relationship.

Answer: False

Explanation: This statement is incorrect. The study indicated that initial infections could be lethal, but prolonged co-evolutionary processes eventually resulted in a stable, mutually dependent relationship.

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The 2024 experiment successfully induced endosymbiosis by implanting bacteria into fungal cells, demonstrating potential changes in the host's DNA.

Answer: True

Explanation: This finding is significant as it demonstrates the experimental induction of endosymbiosis and suggests that such interactions can lead to changes in the host's DNA, offering new avenues for studying the dynamics of symbiosis.

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Zooxanthellae are commonly found in symbiotic relationships with which group of marine organisms?

Answer: Corals and giant clams

Explanation: Zooxanthellae are widely known for their mutualistic symbiosis with reef-building corals and various bivalves, such as giant clams, providing them with photosynthetic products.

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What process do zooxanthellae perform to provide energy to their hosts like corals?

Answer: Photosynthesis

Explanation: Zooxanthellae are photosynthetic algae; they convert light energy into chemical energy in the form of organic compounds, which are then shared with their coral hosts.

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What is notable about the endosymbiotic relationship in *Paulinella chromatophora*?

Answer: The endosymbiont, a cyanobacterium, has evolved into a structure functionally analogous to chloroplasts.

Explanation: The cyanobacterial endosymbiont within *Paulinella chromatophora* has undergone significant evolutionary changes, developing into a specialized organelle termed a chromatophore, which performs photosynthesis, representing a distinct event of organelle evolution.

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The endosymbiotic protists in lower termites are crucial for:

Answer: Digesting lignocellulosic materials.

Explanation: These protists possess the enzymatic machinery necessary to break down cellulose and lignin, complex carbohydrates found in wood and plant matter, thereby allowing termites to utilize these materials as a food source.

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What is the significance of the *Hemialus*-*Richelia* symbiosis for phytoplankton in certain ocean environments?

Answer: *Richelia* fixes atmospheric nitrogen, benefiting *Hemialus* in nitrogen-limited waters.

Explanation: The cyanobacterium *Richelia* possesses the metabolic capability to fix atmospheric nitrogen, a crucial nutrient often scarce in oceanic waters. This nitrogen fixation directly benefits its diatom host, *Hemialus*, supporting its growth and productivity.

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What was significant about the 2024 experiment involving *Rhizopus microsporus* and *Mycetohabitans rhizoxinica*?

Answer: It successfully induced endosymbiosis in a laboratory setting, indicating potential host DNA modifications.

Explanation: This research achieved the experimental induction of endosymbiosis, providing a model system to investigate the molecular mechanisms underlying symbiotic establishment and potential genetic exchanges between host and symbiont.

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